T cells Use Focal Adhesions to Pull Themselves Through Confined Environments

Caillier A, Oleksyn D, Fowell DJ, Miller J, Oakes PW^✉
Submitted (2023)
DOI | bioRχiv | Twitter thread | PMID: 37904911

Smooth Muscle Myosin 2 Filaments Dynamically Assemble and Stabilize During Induced Contractility

Bennet MA, Demeulenaere SG, Wu H, Patel H, Sala S, Longtine ER, Oakes PW, Beach JR^✉
Submitted (2022)
DOI | bioRχiv

α-catenin middle- and actin-binding domain unfolding mutants differentially impact epithelial strength and sheet migration

Quinn JM, Wang Y, Wood M, Flozak AS, Le PM, Yemelyanov A, Oakes PW, Gottardi CJ^✉
Mol Biol Cell In Press (2024)
DOI | bioRχiv | PMID: 38507238

Local Monomer Levels and Established Filaments Potentiate Non-Muscle Myosin 2 Assembly

Quintanilla MA, Patel H, Wu H, Sochacki KA, Chandrasekar S, Akamatsu M, Rotty JD, Korobova F, Bear JE, Taraska JW, Oakes PW^✉, Beach JR^✉
J Cell Biol 223(4):e202305023 (2024)
PDF | DOI | bioRχiv | Twitter thread | PMID: 38353656
- See also: Spotlight: Moonwalking molecular machines: Unraveling the choreography of myosin filament assembly

An interview with Patrick Oakes-Loyola University Chicago, IL, USA

Oakes PW, Trevorrow P
Cytoskeleton (Hoboken), (2024)
PDF | DOI | PMID: 38279969

Machine learning interpretable models of cell mechanics from protein images

Schmitt MS*, Colen J*, Sala S, Devany J, Seetharaman S, Caillier A, Gardel ML^✉, Oakes PW^✉, Vitelli V^✉
Cell, 187(2):481-494 (2024)
PDF | DOI | arΧiv | Twitter thread | PMID: 38194965

Limiting Pool and Actin Architecture Controls Myosin Cluster Sizes in Adherent Cells

Chou W, Molaei M, Wu H, Oakes PW, Beach JR, Gardel ML^✉
Biophys J, 123(2):157-171 (2023)
PDF | DOI | bioRχiv | PMID: 38062704

Measuring response functions of active materials from data

Molaei M*^✉, Redford SA*^✉, Chou W, Scheff D, de Pablo JJ, Oakes PW, Gardel ML^✉
Proc Natl Acad Sci USA, 120(42):e2305283120 (2023)
PDF | DOI | arΧiv | PMID: 37819979

Atypical peripheral actin band formation via overactivation of RhoA and Non-muscle myosin II in Mitofusin 2 deficient cells

Wang Y, Troughton LD, Xu F, Chatterjee A, Zhao H, Cifuentes LP, Wagner RB, Chen J, Kuang S, Suter DM, Yuan C, Chan D, Huang F, Oakes PW, Deng Q^✉
eLife, 12:e88828 (2023)
PDF | DOI | bioRχiv | PMID: 37724949

Shining a Light on RhoA: Optical Control of Cell Contractility

Chandrasekar S, Beach JR, Oakes PW^✉
Int J Biochem Cell Biol, 161:106442 (2023)
PDF | DOI | PMID: 37348811

Repair of Noise-induced Damage to Stereocilia F-actin Cores is Facilitated by XIRP2 and its Novel Mechanosensor Domain

Wagner EL, Im J-S, Sala S, Nakahata MI, Imbery TE, Li S, Chen D, Nimchuk K, Noy Y, Archer DW, Xu W, Hashisaki G, Avraham KB, Oakes PW, Shin J-S^✉
eLife, 12:e72681 (2023)
PDF | DOI | bioRχiv | PMID: 37294664

LIM domain proteins

Sala S, Oakes PW^✉
Curr Biol, 33(9):R339-341 (2023)
PDF | DOI | PMID: 37160086

ST3GAL1 and ΒII-spectrin pathways control CAR T cell migration to target tumors

Hong Y*, Walling BL*, Kim H-R, Serratelli WS, Lozada JR, Sailer CJ, Amitrano AM, Lim K, Mongre RK, Kim K-D, Capece T, Lomakina EB, Reilly NS, Vo K, Gerber SA, Fan T-C, Yu AL, Oakes PW, Waugh RE, Jun C-D, Reagan PM, Kim M^✉
Nat Immunol, 24(6):1007-1019 (2023)
PDF | DOI | PMID: 37069398

Non-muscle myosin 2 filaments are processive in cells

Vitriol EA*^✉, Quintanilla MA*, Tidei JJ*, Troughton LD, Cody A, Cisterna BA, Jane ML, Oakes PW^✉, Beach JR^✉
Biophys J, 122(18):3678-3689 (2023)
PDF | DOI | bioRχiv | PMID: 37218133 | Cover Highlight
- See also: Nonmuscle Myosin 2 Keeps on Truckin’ in this Special Issue Dedicated to Ken Jacobson

Cell scientists to watch – Jordan Beach and Patrick Oakes

J Cell Sci, 136(5):jcs260939 (2023)
PDF | DOI

Quantifying Strain-Sensing Protein Recruitment During Stress Fiber Repair

Seetharaman S*, Sala S*, Gardel ML^✉, Oakes PW^✉
Meth Mol Biol, 2600:169-182 (2023)
PDF | DOI | PMID: 36587097

Meeting report - the ever-fascinating world of septins

Baillet A^✉, McMurray MA^✉, Oakes PW^✉
J Cell Sci, 134(24):jcs259552 (2021)
PDF | DOI | PMID: 34910818

A Workflow for Rapid Unbiased Quantification of Fibrillar Feature Alignment in Biological Images

Marcotti S^✉, Belo de Freitas D, Troughton LD, Kenny FN, Shaw T, Stramer BM, Oakes PW^✉
Front Comput Sci, 3:745831 (2021)
PDF | DOI | bioRχiv | Twitter thread | PMID: 34888522

Stress fiber strain recognition by the LIM protein testin is cryptic and mediated by RhoA

Sala S, Oakes PW^✉
Mol Biol Cell, 32(18):1758-1771 (2021)
PDF | DOI | bioRχiv | Twitter thread | PMID: 34038160

CD4+ T cell interstitial migration controlled by fibronectin in the inflamed skin

Fernandes NR, Reilly NS, Schrock DC, Hocking DC, Oakes PW, Fowell DJ^✉
Front Immunol, 11:1501 (2020)
PDF | DOI | PMID: 32793204

T_RM integrins CD103 and CD49a differentially support adherence and motility after resolution of influenza virus infection

Reilly EC, Lambert-Emo K, Buckley P, Reilly NS, Chaves FA, Yang H, Oakes PW, Topham DJ^✉
Proc Natl Acad Sci USA, 117(22):12306-12314 (2020)
PDF | DOI | bioRχiv | PMID: 32439709 | Cover Highlight

Optogenetic control of RhoA to probe subcellular mechanochemical circuitry

Cavanaugh KE, Oakes PW, Gardel ML^✉
Curr Protoc Cell Biol, 86(1):e102 (2020)
PDF | DOI | PMID: 32031760

Multiscale model of integrin adhesion assembly

Bidone TC, Skeeters AV, Oakes PW, Voth GA^✉
PLoS Comput Biol, 15(6):e1007077 (2019)
PDF | DOI | bioRχiv | PMID: 31163027

Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis

Barger SR, Reilly NS, Shutova MS, Li Q, Maiuri P, Heddleston JM, Mooseker MS, Flavell RA, Svitkina T, Oakes PW, Krendel M^✉, Gauthier NC^✉
Nat Commun, 10:1249 (2019)
PDF | DOI | bioRχiv | PMID: 30890704

Tissue tension and not interphase cell shape determines cell division orientation in the Drosophila follicular epithelium

Finegan TM, Na D, Cammarota C, Skeeters AV, Nádasi TJ, Dawney NS, Fletcher A, Oakes PW, Bergstralh DT^✉
EMBO J, 38(3):e100072 (2019)
PDF | DOI | bioRχiv | PMID: 30478193 | Cover Highlight
- See also: Getting into shape: tissue tension drives oriented cell divisions during organogenesis
- See also: Oriented Cell Division: The Pull of the Pole

Entropy production rate is maximized in non-contractile actomyosin

Seara DS, Yadav V, Linsmeier I, Tabatabai AP, Oakes PW, Tabei SMA, Banerjee S^✉, Murrell  MP^✉
Nat Commun, 9:4948 (2018)
PDF | DOI | arΧiv | PMID: 30470750
- See also: Nature Collection on Active Matter

CCR7 fuels and LFA-1 grips

Oakes PW, Fowell DJ^✉
Nat Immunol, 19(6):516-518 (2018)
PDF | DOI | PMID: 29777210

Balancing forces in migration

Oakes PW^✉
Curr Opin Cell Biol, 54:43-49 (2018)
PDF | DOI | PMID: 29723736

Lamellipodium is a myosin-independent mechanosensor

Oakes PW^✉, Bidone TC, Beckham Y, Skeeters AV, Ramirez-San Juan GR, Winter SP, Voth GA, Gardel ML^✉
Proc Natl Acad Sci USA, 115(11):2646-2651 (2018)
PDF | DOI | bioRχiv | PMID: 294877208

Dia1-dependent adhesions are required by epithelial tissues to initiate invasion

Fessenden TB, Beckham Y, Perez-Neut M, Chourasia AH, Macleod KF, Oakes PW, Gardel ML^✉
J Cell Biol, 217(4):1485-1502 (2018)
PDF | DOI | bioRχiv | PMID: 29437785| Cover Highlight
- See also: JCB Journal Club: Formins are required for invasion
- See also: biosights Podcast: April 2, 2018

Optogenetic control of RhoA reveals zyxin-mediated elasticity of stress fibers

Oakes PW*, Wagner E*, Brand CA, Probst D, Linke M, Schwarz US^✉, Glotzer M^✉, Gardel ML^✉
Nat Commun, 8:15817 (2017)
PDF | DOI | bioRχiv | PMID: 28604737

Cholesterol depletion impairs contractile machinery in neonatal rat cardiomyocytes

Hissa B, Oakes PW, Pontes B, Ramirez-San Juan GR, Gardel ML^✉
Sci Rep, 7:43764 (2017)
PDF | DOI | PMID: 28256617

Contact guidance requires spatial control of leading edge protrusion

Ramirez-San Juan GR, Oakes PW, Gardel ML^✉
Mol Biol Cell, 28(8):1043-1053 (2017)
PDF | DOI | PMID: 28228548

A coin vibrational motor swimming at low reynolds number

Quillen AC^✉, Askari H, Kelley DH, Friedmann T, Oakes PW
Regul Chaotic Dyn, 21:902-917 (2016)
PDF | DOI | arΧiv

Disordered actomyosin networks are sufficient to produce cooperative and telescopic contractility

Linsmeier I, Banerjee S, Oakes PW, Jung W, Kim T, Murrell MP^✉
Nat Commun, 7:12615 (2016)
PDF | DOI | PMID: 27558758

Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton

Bridges AA, Jentzsch MS, Oakes PW, Occhipinti P, Gladfelter AS^✉
J Cell Biol, 213(1):23-32 (2016)
PDF | DOI | PMID: 27044896
- See Also: Septins recognize micron-scale membrane curvature

Measuring cell mechanics

Gardel M^✉, Oakes P^✉
Colloquium Series on Quantitative Cell Biology: Vol. 3
Marshall WF, ed. Morgan & Claypool Life Science Publishers. (2015)
PDF | DOI

Forcing cells into shape: the mechanics of actomyosin contractility

Murrell M, Oakes PW, Lenz M, Gardel ML^✉
Nat Rev Mol Cell Biol, 16(8):486-498 (2015)
PDF | DOI | PMID: 26130009

Model-based traction force microscopy reveals differential tension in cellular actin bundles

Soiné JRD*, Brand CA*, Stricker J, Oakes PW, Gardel ML, Schwarz US^✉
PLoS Comput Biol, 11(3):e1004076 (2015)
PDF | DOI | PMID: 25748431

Epithelial rotation promotes the global alignment of contractile actin bundles during Drosophila egg chamber elongation

Cetera M*, Ramirez-San Juan GR*, Oakes PW, Lewellyn L, Fairchild MJ, Tanentzapf G, Gardel ML, Horne-Badovinac S^✉
Nat Commun, 5:5511 (2014)
PDF | DOI | PMID: 25413675

Geometry regulates traction stresses in adherent cells

Oakes PW, Banerjee S, Marchetti CM, Gardel ML^✉
Biophys J, 107(4):825-833 (2014)
PDF | DOI | PMID: 25140417 | Cover Highlight
- See also: Work and tension: new evidence that adherent cells of same area do the same work independent of stiffness and focal adhesions

Stressing the limits of focal adhesion mechanosensitivity

Oakes PW, Gardel ML^✉
Curr Opin Cell Biol, 30:68-73 (2014)
PDF | DOI | PMID: 24998185

Actin assembly factors regulate the gelation kinetics and architecture of F-actin networks

Falzone TT, Oakes PW, Sees J, Kovar D, Gardel ML^✉
Biophys J, 104(8):1709-1719 (2013)
PDF | DOI | PMID: 23601318

Tension is required but not sufficient for focal adhesion maturation without a stress fiber template

Oakes PW*, Beckham Y*, Stricker J, Gardel ML^✉
J Cell Biol, 196(3):363-374 (2012)
PDF | DOI | PMID: 22291038 | Cover Highlight
- See also: In focus: stress fibers guide focal adhesions to maturity
- Selected for F1000

Spatiotemporal constraints on the force-dependent growth of focal adhesions

Stricker J, Aratyn-Schaus Y, Oakes PW, Gardel ML^✉
Biophys J, 100(12):2883-93 (2011)
PDF | DOI | PMID: 21689521

Dynamic and structural signatures of lamellar actomyosin force generation

Aratyn-Schaus Y, Oakes PW, Gardel ML^✉
Mol Biol Cell, 22(8):1330-1339 (2011)
PDF | DOI | PMID: 21307339 | Cover Highlight
- See also: Highlights from MBoC

Preparation of compliant matrices for quantifying cellular contraction

Aratyn-Schaus Y, Oakes PW, Stricker J, Winter SP, Gardel ML^✉
J Vis Exp, Dec 14 (46) e2173 (2010)
PDF | DOI | PMID: 21178972 | Video

Neutrophil morphology and migration are affected by substrate stiffness

Oakes PW, Patel DC, Morin NA, Zitterbart DP, Fabry B, Reichner JS^✉, Tang JX
Blood, 114:1387-1395 (2009)
PDF | DOI | PMID:19491394

Nonmuscle myosin heavy chain IIA mediates integrin LFA-1 de-adhesion during T lymphocyte migration

Morin NA, Oakes PW, Hyun Y-M, Lee D, Chin EY, King MR, Springer TA, Shimaoka M, Tang JX, Reichner JS, Kim M^✉
J Exp Med, 205(1):195-205 (2008)
PDF | DOI | PMID:18195072

Growth of tactoidal droplets during the first-order isotropic to nematic phase transition of F-actin

Oakes PW, Viamontes J, Tang JX^✉
Phys Rev E, 75(6):061902-11 (2007)
PDF | DOI | PMID: 17677295

Isotropic to nematic liquid crystalline phase transition of F-actin varies from continuous to first order

Viamontes J, Oakes PW, Tang JX^✉
Phys Rev Lett, 97(11):118103 (2006)
PDF | DOI | arΧiv | PMID: 17025933